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Research Methods
-Optimized Method for Harvesting:
For optimal results, the media in the flask was dumped out and replaces with new media. Then, the flask was shaken to stimulate a turbulent river environment that the cells best flourish in.
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Step 1: Once the old media is dumped, 150 mL of new media is measured out.
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Step 2: Autoclaved spring water is added to the media until the mixture is up to 500mL.
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Step 3: The new media is then put back into the flask with cells, and then shaken overnight at 80 rpm.
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Step 4: A cheese cloth is secures on a Whattman pipe.
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Step 5: The Whatmann is placed on the funnel and the shaken media is poured through slowly.
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Step 6: A petri dish with spring water is retrieved; the cells clinging onto the cheese cloth will detach and attach onto the bottom of the petri dish.
-Optimized Method for Image Processing:
With the use of ImageJ, I was able to turn the shown trajectory of a cell into a set of data that can be processed. Below is the steps taken to get those data. From there, MATLAB was used to make the figures.
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